Mammalian Synthetic Promoters by David Gould

Author:David Gould
Language: eng
Format: epub
Publisher: Springer New York, New York, NY

3.If the pool of identified TFREs is relatively large (e.g., >30), a filtering step can be applied to minimize the design space. For example, by only selecting TFREs that occur in at least two different promoters.

4.Select a “useable” sequence for each identified TFRE. This can be the sequence as it most commonly occurred in analyzed promoters or a consensus sequence derived from a position weight matrix (available in databases such as JASPAR: http://​jaspar.​genereg.​net/​ [15] (see Note 4).

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